Journal: The Journal of Biological Chemistry

Article Title: The prostaglandin E2 receptor EP3 controls CC-chemokine ligand 2–mediated neuropathic pain induced by mechanical nerve damage

doi: 10.1074/jbc.RA118.002492

Figure Lengend Snippet: cPGES but not mPGES-1 is expressed in macrophages at the injury site. A, LC–MS/MS analysis of PGE2 in the ipsi- and contralateral sciatic nerves 10 days after the SNI operation. The data are shown as means ± S.E. (n = 6). **, p ≤ 0.01 one-tailed t test. B, representative Western blotting for cPGES and mPGES-1 in ipsilateral and contralateral sciatic nerves 10 days after the SNI operation; bone marrow–derived macrophages stimulated for 24 h with 0.1 μg of LPS served as positive control. C, densitometric analysis of four independent Western blotting experiments as shown in B. The data are shown as means ± S.E. **, p ≤ 0.01, two-tailed t test. D and E, MELC analysis of sciatic nerve 10 days after the SNI operation. The white squares indicate the regions shown for cPGES, IBA1, CD11b, CD45, and F4-80. White arrows indicate cells expressing these five markers. The radar chart (E) displays the percentage of cPGES-expressing cells (100%) that are positive for the respective stated marker (n = 40).

Article Snippet: This was followed by incubation with an antibody mixture for 30 min on ice comprising antibodies listed in or for multiparameter FACS analysis CD45-Vioblue, HLA-DR-APC (Miltenyi Biotec, Bergisch-Gladbach, Germany), F4/80-PE-Cy7 (eBioscience, Frankfurt, Germany), CD3-PE-CF594, CD11b-BV605, CD11c-AlexaFluor700, CD19-APC-H7, Ly6C-PerCP-Cy5.5, and Ly6G-APC–Cy7, NK1.1-PE (BD Biosciences).

Techniques: Liquid Chromatography with Mass Spectroscopy, One-tailed Test, Western Blot, Derivative Assay, Positive Control, Two Tailed Test, Expressing, Marker

Journal: The Journal of Biological Chemistry

Article Title: The prostaglandin E2 receptor EP3 controls CC-chemokine ligand 2–mediated neuropathic pain induced by mechanical nerve damage

doi: 10.1074/jbc.RA118.002492

Figure Lengend Snippet: Immune cell recruitment to the site of injury is decreased in EP3−/− mice. A and B, FACS analysis with cells isolated from the site of injury from WT and EP3−/− mice 10 days after the SNI operation. The gating strategy is shown in A. The data are shown as means ± S.E. (WT, n = 7; EP3−/− ipsilateral, n = 6; EP3−/− contralateral, n = 5; two-way ANOVA/Bonferoni post hoc test). *,#, p ≤ 0.05; **,##, p ≤ 0.01; ***, p < 0.001. #, compared with contralateral.

Article Snippet: This was followed by incubation with an antibody mixture for 30 min on ice comprising antibodies listed in or for multiparameter FACS analysis CD45-Vioblue, HLA-DR-APC (Miltenyi Biotec, Bergisch-Gladbach, Germany), F4/80-PE-Cy7 (eBioscience, Frankfurt, Germany), CD3-PE-CF594, CD11b-BV605, CD11c-AlexaFluor700, CD19-APC-H7, Ly6C-PerCP-Cy5.5, and Ly6G-APC–Cy7, NK1.1-PE (BD Biosciences).

Techniques: Isolation

Journal: The Journal of Biological Chemistry

Article Title: The prostaglandin E2 receptor EP3 controls CC-chemokine ligand 2–mediated neuropathic pain induced by mechanical nerve damage

doi: 10.1074/jbc.RA118.002492

Figure Lengend Snippet: EP3-deficiency on myeloid cells does not affect mechanical allodynia or immune cell recruitment in the SNI model. A, genotyping of white blood cells from irradiated WT mice having received bone marrow from wild-type (WT/WT) or EP3−/− (EP3−/−/WT) mice. The lower PCR band (450 bp) shows WT and the upper and 650-bp EP3 knockouts. B, C57Bl/6N received bone marrow from either WT or EP3-deficient mice before they underwent SNI surgery. Mechanical allodynia was determined before and at the indicated times after surgery. The data are shown as means ± S.E. (WT, n = 7; EP3−/−, n = 10). C, immune cell numbers determined by FACS analysis at the site of injury at the sciatic nerve in bone marrow–transplanted mice. The data are shown as means ± S.E. (WT, n = 5; EP3−/−, n = 7).

Article Snippet: This was followed by incubation with an antibody mixture for 30 min on ice comprising antibodies listed in or for multiparameter FACS analysis CD45-Vioblue, HLA-DR-APC (Miltenyi Biotec, Bergisch-Gladbach, Germany), F4/80-PE-Cy7 (eBioscience, Frankfurt, Germany), CD3-PE-CF594, CD11b-BV605, CD11c-AlexaFluor700, CD19-APC-H7, Ly6C-PerCP-Cy5.5, and Ly6G-APC–Cy7, NK1.1-PE (BD Biosciences).

Techniques: Irradiation

Journal: The Journal of Biological Chemistry

Article Title: The prostaglandin E2 receptor EP3 controls CC-chemokine ligand 2–mediated neuropathic pain induced by mechanical nerve damage

doi: 10.1074/jbc.RA118.002492

Figure Lengend Snippet: EP3 activation induces the release of CCL2 from DRG neurons and mast cells. A, CCL2 release from DRG cultures of WT and EP3−/− mice was determined by ELISA after 6 h of incubation with vehicle, sulprostone (1 μg/ml), or LPS (25 ng/ml). The data are shown as means ± S.E. (WT, n = 8; EP3−/−, n = 5). *, p ≤ 0.05; Student's t test. B, CCL2 release from mast cells of WT mice was determined by ELISA after 6 h of incubation with vehicle, sulprostone or PMA (0.1 μm). The data are shown as means ± S.E. (n = 5). **, p ≤ 0.01; ***, p ≤ 0.001; ***, p ≤ 0.001; one-way ANOVA with Bonferroni multiple comparison. C, CCL2 release from mast cells of EP3−/− mice was determined by ELISA after 6 h incubation with vehicle, sulprostone (1 μg/ml), or PMA (0.1 μm). The data are shown as means ± S.E. (n = 5). ***, p ≤ 0.001; one-way ANOVA with Bonferroni multiple comparison. D, FACS analysis with cells isolated from the site of injury from WT and Mcpt5–DTA–Cre−/+ mice 14 days after the SNI operation. The data are shown as means ± S.E. (n = 5). *, p ≤ 0.05; **, p ≤ 0.01; ***, p < 0.001; two-way ANOVA/Bonferoni post hoc test. E, Cre-positive and -negative Mcpt5 DTA mice underwent SNI surgery. Mechanical thresholds were measured before surgery and at the indicated times after SNI. The data are shown as means with ± S.E. (n = 6). PWL, paw withdrawal latency.

Article Snippet: This was followed by incubation with an antibody mixture for 30 min on ice comprising antibodies listed in or for multiparameter FACS analysis CD45-Vioblue, HLA-DR-APC (Miltenyi Biotec, Bergisch-Gladbach, Germany), F4/80-PE-Cy7 (eBioscience, Frankfurt, Germany), CD3-PE-CF594, CD11b-BV605, CD11c-AlexaFluor700, CD19-APC-H7, Ly6C-PerCP-Cy5.5, and Ly6G-APC–Cy7, NK1.1-PE (BD Biosciences).

Techniques: Activation Assay, Enzyme-linked Immunosorbent Assay, Incubation, Comparison, Isolation

Journal: The Journal of Biological Chemistry

Article Title: The prostaglandin E2 receptor EP3 controls CC-chemokine ligand 2–mediated neuropathic pain induced by mechanical nerve damage

doi: 10.1074/jbc.RA118.002492

Figure Lengend Snippet: Antibodies used for MELC and FACS analysis 7-AAD, 7-aminoactinomycin D; MHC, major histocompatibility complex.

Article Snippet: This was followed by incubation with an antibody mixture for 30 min on ice comprising antibodies listed in or for multiparameter FACS analysis CD45-Vioblue, HLA-DR-APC (Miltenyi Biotec, Bergisch-Gladbach, Germany), F4/80-PE-Cy7 (eBioscience, Frankfurt, Germany), CD3-PE-CF594, CD11b-BV605, CD11c-AlexaFluor700, CD19-APC-H7, Ly6C-PerCP-Cy5.5, and Ly6G-APC–Cy7, NK1.1-PE (BD Biosciences).

Techniques:

Journal: Journal of Clinical Medicine

Article Title: How Cardiac Fibrosis Assessed via T1 Mapping Is Associated with Liver Fibrosis in Patients with Non-Alcoholic Fatty Liver Disease

doi: 10.3390/jcm12237381

Figure Lengend Snippet: Diagram of study flow. CIMT, carotid intima–media thickness; MRI, magnetic resonance imaging.

Article Snippet: Multiparametric resonance studies were performed on a 3.0 T MRI system (Magnetom Prisma; Siemens Healthcare, Erlangen, Germany) using a combined eighteen-element phased-array abdominal coil and a thirty-two-element fixed spine coil.

Techniques: Magnetic Resonance Imaging

Journal: Journal of Clinical Medicine

Article Title: How Cardiac Fibrosis Assessed via T1 Mapping Is Associated with Liver Fibrosis in Patients with Non-Alcoholic Fatty Liver Disease

doi: 10.3390/jcm12237381

Figure Lengend Snippet: Visual summary illustrating the association between cardiac extracellular volume (ECV) measured via MRI and liver fibrosis detected with magnetic resonance elastography (MRE) in patients with non-alcoholic fatty liver disease (NAFLD).

Article Snippet: Multiparametric resonance studies were performed on a 3.0 T MRI system (Magnetom Prisma; Siemens Healthcare, Erlangen, Germany) using a combined eighteen-element phased-array abdominal coil and a thirty-two-element fixed spine coil.

Techniques:

Journal: Journal of Clinical Medicine

Article Title: How Cardiac Fibrosis Assessed via T1 Mapping Is Associated with Liver Fibrosis in Patients with Non-Alcoholic Fatty Liver Disease

doi: 10.3390/jcm12237381

Figure Lengend Snippet: Liver magnetic resonance elastography (MRE) of two patients, one without ( A – C ) and the other with ( D – F ) significant liver fibrosis. ( A , D ): Conventional abdominal out-of-phase T1-weighted MRI images show normal liver contour with steatosis ( A ) and irregular liver contour, indicating chronic liver disease ( D ). ( B , E ): Wave images from MRE acquisition depicting thin waves ( B ) in the patient with no fibrosis and thicker waves ( E ) in the patient with significant liver fibrosis. ( C , F ): Corresponding color elastograms with 95% of confidence interval in the map show normal liver stiffness values in the patient with no significant fibrosis ( C ) and elevated liver stiffness values in the patient with significant fibrosis ( F ).

Article Snippet: Multiparametric resonance studies were performed on a 3.0 T MRI system (Magnetom Prisma; Siemens Healthcare, Erlangen, Germany) using a combined eighteen-element phased-array abdominal coil and a thirty-two-element fixed spine coil.

Techniques: